Wolters G, Kuijpers L P, Croese J
J Virol Methods. 1980 Dec;2(1-2):57-62. doi: 10.1016/0166-0934(80)90039-7.
Two modifications of Hepanostika were studied in order to improve this enzyme-immunoassay with regard to duration of the test, sensitivity and reading of test results. Test conditions and composition of some components of the test system were modified. With the first modification the test could be performed within 3 h and was about 3X as sensitive as Hepanostika. However, this method was less specific than Hepanostika and it was not suitable for routine screening. A second modification was studied with two differently prepared conjugates. The duration of the test was less than 4 h and the sensitivity was at least twice that of Hepanostika. The test results could be measured directly with a suitable photometer. Preliminary results with more than 500 deep-frozen donor sera showed that the specificity was acceptable with a number of false positives being less than 2%.
为了在检测时间、灵敏度和检测结果判读方面改进这种酶免疫测定法，对两种改良的肝酶谱测定法进行了研究。对检测条件和检测系统某些组分的组成进行了改良。第一种改良方法可在3小时内完成检测，灵敏度约为肝酶谱测定法的3倍。然而，这种方法的特异性低于肝酶谱测定法，不适合常规筛查。对第二种改良方法使用了两种不同制备的结合物进行研究。检测时间少于4小时，灵敏度至少是肝酶谱测定法的两倍。检测结果可用合适的光度计直接测定。对500多份深冻供体血清的初步检测结果表明，特异性是可接受的，假阳性数量少于2%。
