Meola J M, Rosano T G, Swift T A
Clin Chem. 1980 Nov;26(12):1709.
We describe an assay for ethylene glycol in serum, which is based on the Hantzsch condensation reaction. Ethylene glycol is dehydrogenated by sodium periodate to form two molecules of formaldehyde, which is then combined with ammonium ion and acetylacetone to form a fluorescent diacetyllutidine derivative. The detection limit of this method is 50 mg/L. The procedure is sensitive, requires only 100 micro L of serum, and the standard curve is linear over a working range of 50 to 250 mg/L. Average analytical recovery ranged from 99 to 102% and within-run precision studies showed CVs of < 5%. Reagents are easily prepared and available.
我们描述了一种基于汉茨希缩合反应的血清中乙二醇检测方法。乙二醇被高碘酸钠脱氢形成两分子甲醛，然后甲醛与铵离子和乙酰丙酮结合形成荧光二乙酰卢剔啶衍生物。该方法的检测限为50mg/L。该方法灵敏，仅需100微升血清，标准曲线在50至250mg/L的工作范围内呈线性。平均分析回收率在99%至102%之间，批内精密度研究显示变异系数<5%。试剂易于制备且可得。
