Simon O, Adam K, Bergner H
Arch Tierernahr. 1978 Sep;28(9):609-17. doi: 10.1080/17450397809424599.
Mature male albino rats (ca. 300 g body mass) received 10 diets with a varying lysine content (1.6 to 8.4 g/16 g N). In one partial test the animals were fed ad libitum and in another partial test they were kept in the state of maintenance by limiting the amount of the diet. After 7 feeding days the catabolisation of 14C-lysine into 14CO2 was measured and after 8 feeding days the 15N-excretion in urine after 15N-lysine doses was ascertained. Based on these characteristics typical of metabolism, which show increased catabolisation of the amino acid after the lysine requirement was met, the lysine requirement of a mature rat was determined as 3.5 to 4.0 g/16 g N in the diet. In conclusion, this shows that the lysine requirement in the state of maintenenace and of mature animals is considerably lower than during the period of growth.
成年雄性白化大鼠（体重约300克）接受了10种赖氨酸含量不同（1.6至8.4克/16克氮）的日粮。在一项部分试验中，动物自由采食，在另一项部分试验中，通过限制日粮量使动物维持在维持状态。喂养7天后，测定14C-赖氨酸分解代谢为14CO2的情况，喂养8天后，确定给予15N-赖氨酸剂量后尿液中的15N排泄量。基于这些典型的代谢特征，即赖氨酸需求得到满足后氨基酸分解代谢增加，确定成年大鼠日粮中赖氨酸的需求量为3.5至4.0克/16克氮。总之，这表明维持状态下的成年动物对赖氨酸的需求量远低于生长时期。
