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[组织学实践中半薄环氧树脂切片的处理方法]

[Methods for working with semi-thin epoxide sections in histological practice].

作者信息

Kostilenko Iu P, Kovalev E V

出版信息

Arkh Anat Gistol Embriol. 1978 Dec;75(12):68-72.

PMID:742988
Abstract

Since ordinary histological laboratories have no special equipment, semi-thin epoxide embedded sections, to perfectly suitable to realize the resolving capacity of the light optic are not widely used in histological practice. However, even in an ordinary histological laboratory it is possible to master the method of their production. A special device is suggested to fix glass knives in the rotational microtome MPC-2. Simultaneously, a number of suggestions and recommendations are given as to certain specificities for obtaining serial semi-thin sections and the method for their staining. Besides, an original method is offered for producing film preparations by means of mild tissue compression in the process of polymerization in epoxide resin. This method allows not only to study some features in the composition of complex three-dimentional objects, but to obtain a series of semi-thin sections from the film preparation to reveal intimal interconnections between certain structural components.

摘要

由于普通组织学实验室没有特殊设备,完全适合实现光学显微镜分辨能力的半薄环氧树脂包埋切片在组织学实践中并未得到广泛应用。然而,即使在普通组织学实验室也能够掌握其制作方法。建议使用一种特殊装置将玻璃刀固定在MPC - 2旋转切片机上。同时,针对获取连续半薄切片的某些特殊性及其染色方法给出了一些建议和推荐。此外,还提供了一种原始方法,即在环氧树脂聚合过程中通过轻度组织压缩来制作薄膜标本。这种方法不仅能够研究复杂三维物体组成中的一些特征,还能从薄膜标本上获取一系列半薄切片以揭示某些结构成分之间的内膜连接。

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