Williams T, Rogers A W
J Anat. 1980 Jun;130(Pt 4):867-81.
The luminal epithelium of the uterus has been examined morphometrically in ovariectomized rats from 0--25 hours after a single injection of either 2.5 mg progesterone or 2.5 micrograms oestradiol subcutaneously. In each case, the epithelial cells were 50% taller at 25 hours than in controls. In progesterone series, this growth occurred between 6 and 25 hours: it was preceded by a significant increase in nuclear size and in the volume fraction of cytoplasm occupied by free ribosomes at 3 hours, and increases in the volume fractions of free ribosomes and RER at 6 hours. AT 25 hours, many smooth-surfaced vesicles appeared at the cell apex. In the oestradiol series, cell size decreased at 3 and 6 hours before increasing at 25 hours. Apical vesicles were reduced in number at all times after oestradiol: at 25 hours the nucleolus showed a fivefold increase in size, the RER had a greater volume fraction, and the lipid droplets were significantly reduced in number. The nature and timing of these results are consistent with the hypothesis that progesterone acts on these cells by a different mechanism to oestradiol, which does not involve DNA-directed RNA synthesis.
在皮下单次注射2.5毫克孕酮或2.5微克雌二醇后的0至25小时内，对去卵巢大鼠的子宫腔上皮进行了形态计量学检查。在每种情况下，25小时时上皮细胞比对照组高50%。在孕酮组中，这种生长发生在6至25小时之间：在3小时时，核大小和游离核糖体占据的细胞质体积分数显著增加之前就已开始，在6小时时，游离核糖体和粗面内质网的体积分数增加。在25小时时，细胞顶端出现许多表面光滑的小泡。在雌二醇组中，细胞大小在3小时和6小时时减小，然后在25小时时增加。雌二醇处理后，顶端小泡数量在所有时间都减少：在25小时时，核仁大小增加了五倍，粗面内质网的体积分数更大，脂滴数量显著减少。这些结果的性质和时间与以下假设一致，即孕酮对这些细胞的作用机制与雌二醇不同，雌二醇不涉及DNA指导的RNA合成。
