Severance P J, Kauffman C A, Sheagren J N
J Clin Microbiol. 1980 Jun;11(6):724-7. doi: 10.1128/jcm.11.6.724-727.1980.
Lysostaphin sensitivity was evaluated as a rapid screening test to differentiate Staphylococcus aureus from other species of staphylococci and micrococci. A total of 168 strains of staphylococci, 108 of which were S. aureus, were cultured overnight in brain infusion broth. Gram stains were peformed before and after a 1:10 dilution of the culture was exposed to 2 micrograms of lysostaphin per ml at 37 degrees C for 30 min. A reduction of 90% or greater in the number of organisms seen on comparison of the pre- and posttreatment Gram stains was considered a "positive" test result and was found in 106 of 108 S. aureus strains; 60 of 60 non-S. aureus staphylococci had a negative test result, showing no difference between the pre- and posttreatment Gram stains. Identical results were obtained using commerical blood culture media in place of brain heart infusion broth. Also studied prospectively were 100 blood or broth cultures which the clinical microbiology laboratory identified as containing gram-positive cocci suggestive of staphylococci. All 33 cultures later found to contain S. aureus gave positive test results; 67 of 67 non-S. aureus staphylococci, micrococci, and steptococci were negative.
溶葡萄球菌素敏感性被评估为一种快速筛选试验，用于区分金黄色葡萄球菌与其他葡萄球菌和微球菌物种。总共168株葡萄球菌，其中108株为金黄色葡萄球菌，在脑浸液肉汤中过夜培养。在将培养物按1:10稀释后，于37℃下暴露于每毫升含2微克溶葡萄球菌素的溶液中30分钟，前后分别进行革兰氏染色。与处理前和处理后的革兰氏染色相比，观察到的菌数减少90%或更多被视为“阳性”试验结果，在108株金黄色葡萄球菌菌株中有106株出现该结果；60株非金黄色葡萄球菌的葡萄球菌试验结果为阴性，处理前和处理后的革兰氏染色无差异。使用商业血培养培养基代替脑心浸液肉汤也获得了相同的结果。还对临床微生物学实验室鉴定为含有提示葡萄球菌的革兰氏阳性球菌的100份血液或肉汤培养物进行了前瞻性研究。后来发现所有33份含有金黄色葡萄球菌的培养物试验结果均为阳性；67份非金黄色葡萄球菌的葡萄球菌、微球菌和链球菌培养物试验结果均为阴性。
