鉴定葡萄球菌属和微球菌属方法的比较
Comparison of methods for identifying Staphylococcus and Micrococcus spp.
作者信息
Gunn B A, Singleton F L, Peele E R, Colwell R R, Keiser J K, Kapper C O
出版信息
J Clin Microbiol. 1981 Aug;14(2):195-200. doi: 10.1128/jcm.14.2.195-200.1981.
Abstract
Three methods employed to distinguish staphylococci from micrococci were compared, using clinical and environmental strains. When these methods are used, misinterpretation of results, as well as erratic results, may occur, and suggestions for eliminating these problems are provided. The most sensitive test that combines ease of use and speed in obtaining results for distinguishing the two genera is the lysostaphin susceptibility test. Two other tests, facultatively anaerobic growth in semisolid thioglycolate agar and fermentation of dextrose, may also be used to distinguish these two genera, but results are often slow in developing, are subject to technical difficulties, and may lead to incorrect assignment of certain species of staphylococci and micrococci to their proper genera.
摘要
采用临床菌株和环境菌株，比较了三种区分葡萄球菌和微球菌的方法。使用这些方法时，可能会出现结果误判以及结果不稳定的情况，并针对消除这些问题提供了建议。在区分这两个菌属时，结合了易用性和快速获得结果的最灵敏测试是溶葡萄球菌素敏感性测试。另外两种测试，即在半固体硫代乙醇酸盐琼脂中兼性厌氧生长和葡萄糖发酵，也可用于区分这两个菌属，但结果通常出现较慢，存在技术难题，并且可能导致某些葡萄球菌和微球菌物种被错误归类到它们所属的菌属。
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