一种新的中性粒细胞念珠菌杀伤试验：季也蒙念珠菌的铬-51释放试验
A new neutrophil candida killing test: chromium-51 release from Candida guilliermondii.
作者信息
Martin S, Ghoneim A T, Child J A
出版信息
J Clin Pathol. 1980 Aug;33(8):757-61. doi: 10.1136/jcp.33.8.757.
Neutrophil candidacidal function was measured by a chromium-51 (51Cr) release technique, using Candida guilliermondii as the test organism. The effect of varying the neutrophil:candida ratio, the time course of 51Cr release, the effect of different serum concentrations, and the reproducibility of the technique were investigated. Results from 33 experiments, using neutrophils from 35 normal donors and six patients with known neutrophil defects, show the technique to be a reliable and reproducible assay of candida killing. The chromium release assay has the advantage of being easy to perform and not liable to subjective errors.
采用铬-51（51Cr）释放技术，以季也蒙念珠菌作为测试微生物，测定中性粒细胞的杀念珠菌功能。研究了改变中性粒细胞与念珠菌比例的影响、51Cr释放的时间进程、不同血清浓度的影响以及该技术的可重复性。来自33项实验的结果，使用了35名正常供体和6名已知中性粒细胞缺陷患者的中性粒细胞，表明该技术是一种可靠且可重复的念珠菌杀伤检测方法。铬释放检测法具有操作简便且不易产生主观误差的优点。
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