Yamamura M, Boler J, Valdimarsson H
J Immunol Methods. 1976;13(3-4):227-33. doi: 10.1016/0022-1759(76)90069-7.
Intracellular killing of Candida albicans was measured by a chromium release technique. Appropriate conditons were equal numbers of polymorphonuclear leucocytes (PMN) and 51Chromium labelled C. albicans (10 6/ml), fresh plasma at a final concentration of 2.5%, incubated at 37degreesC for 60 min. Using normal PMNs, 35-71% of releasable chromium was liberated into the supernatant under these conditions. This assay is easy to perform, requires a small amount of blood and offers and objective measurement of intracellular killing of C. albicans.
采用铬释放技术测定白色念珠菌的细胞内杀伤情况。合适的条件为：多形核白细胞（PMN）数量与51铬标记的白色念珠菌数量相等（10⁶/ml），新鲜血浆终浓度为2.5%，于37℃孵育60分钟。在这些条件下，使用正常PMN时，35% - 71%可释放的铬释放到上清液中。该检测方法易于操作，所需血量少，且能客观测量白色念珠菌的细胞内杀伤情况。
