Uldall A
J Clin Chem Clin Biochem. 1980 May;18(5):303-4.
A simplification of the borohydride-cyanonitrosylferrate (nitroprusside) test for cystine in urine is described. The borohydride reagent is stabilised by cooling and by using pure components. Octanol-1 is used to reduce foaming. The cyanonitrosylferrate reagent is stabilised by sulphite and by storage at -80 degrees C. Compared with previous methods, it is possible to use higher (more than 4 times) dilutions of urine. An aqueous cystine solution may be used as a standard for visual evaluation; for the previous methods there is no well defined material for standardisation. The described procedure is intended for visual evaluation; the method is also suitable for quantitation by photometry.
本文描述了一种用于检测尿中胱氨酸的硼氢化物-氰基亚硝酰铁（硝普钠）试验的简化方法。硼氢化物试剂通过冷却和使用纯组分来实现稳定。正辛醇-1用于减少泡沫。氰基亚硝酰铁试剂通过亚硫酸盐和在-80℃下储存来实现稳定。与以前的方法相比，可以使用更高（超过4倍）稀释度的尿液。可以使用胱氨酸水溶液作为目视评估的标准；对于以前的方法，没有明确界定的标准化物质。所描述的程序用于目视评估；该方法也适用于通过光度法进行定量分析。
