Klein R A, Halliday D, Pittet P G
Lipids. 1980 Aug;15(8):572-9. doi: 10.1007/BF02534181.
We show in this paper that 13-methyltetradecanoic acid (13-MTD) can be used as a structually labeled marker for investigating the mobility of fatty acyl chains in adipose tissue in the rat. The presence of an omega-1 methyl group allows easy quantitation by gas liquid chromatography (GLC) and permits an assessment to be made of any oxidation and chain elongation reactions with reincorporation of the label into the adipose tissue, since the iso-acyl chain is well resolved from odd or even-numbered homologous fatty acids with straight chains. The kinetics of uptake and loss of the structural label were different for adipose tissue taken from the various sites which were sampled, namely post abdominal, mesenteric, perirenal, pericardiac and subcutaneous adipose tissue as well as the epidydimal fat pads. We also report preliminary results in man which confirm that the method is applicable to human clinical studies and that 13-MTD kinetics differ for adipose tissue taken from the 3 different subcutaneous sites--waist, arm and thigh.
我们在本文中表明，13-甲基十四烷酸（13-MTD）可作为一种结构标记物，用于研究大鼠脂肪组织中脂肪酰链的流动性。ω-1甲基的存在使得通过气相色谱法（GLC）能够轻松进行定量分析，并且可以评估任何氧化和链延长反应，以及标记物重新掺入脂肪组织的情况，因为异酰链与具有直链的奇数或偶数同源脂肪酸能够很好地分离。对于从不同采样部位获取的脂肪组织，即腹后、肠系膜、肾周、心包和皮下脂肪组织以及附睾脂肪垫，结构标记物的摄取和丢失动力学是不同的。我们还报告了在人体中的初步结果，证实该方法适用于人体临床研究，并且从三个不同皮下部位——腰部、手臂和大腿获取的脂肪组织中，13-MTD的动力学存在差异。
