Pittet P G, Bessart T, Jéquier E, Philippossian G, Liardon R
Int J Vitam Nutr Res. 1983;53(1):115-9.
Three healthy male subjects had their adipose tissue enriched with a structurally-labelled fatty acid, 13-methyltetradecanoic acid (13-MTD), occurring naturally only in trace amounts. Needle biopsies from femoral, gluteal and abdominal adipose tissue were obtained at several intervals during a 6-week 13-MTD ingestion period and also along an 11-week period following the cessation of the label intake. Fatty acid composition was analysed by gas-liquid chromatography. Using this method, it was possible to show that different adipose tissues had various responsiveness to labelling (with abdominal fat having the highest one) and hence had different turnover rates. Incorporation and particularly disappearance rates were slow, half-lifes for label incorporation ranged from 2 to 12 weeks and that for label mobilization ranged from 4 to 10 months, thus confirming few other published results. The unexpected observation that incorporation of the label did not cease at the end of the 13-MTD feeding period, but did continue for at least a fortnight, might indicate that existence of a buffer pool where fatty acids may be momentarily stored before their definite incorporation into adipose tissue, as suggested in few other studies.
三名健康男性受试者的脂肪组织中富集了一种结构标记的脂肪酸——13-甲基十四烷酸（13-MTD），这种脂肪酸仅以痕量天然存在。在为期6周的13-MTD摄入期内的几个时间点，以及在停止标记物摄入后的11周内，从股骨、臀和腹部脂肪组织获取针吸活检样本。通过气液色谱法分析脂肪酸组成。使用这种方法，可以表明不同的脂肪组织对标记的反应不同（腹部脂肪的反应最高），因此具有不同的周转率。掺入率，尤其是消失率较慢，标记掺入的半衰期为2至12周，标记动员的半衰期为4至10个月，从而证实了其他一些已发表的结果。意外的观察结果是，在13-MTD喂养期结束时，标记的掺入并未停止，而是至少持续了两周，这可能表明存在一个缓冲池，脂肪酸在确定掺入脂肪组织之前可能会暂时储存在其中，其他一些研究也有类似的推测。
