电离辐射对共济失调毛细血管扩张症细胞中DNA合成的影响。
Effect of ionizing radiation on DNA synthesis in ataxia telangiectasia cells.
作者信息
Houldsworth J, Lavin M F
出版信息
Nucleic Acids Res. 1980 Aug 25;8(16):3709-20. doi: 10.1093/nar/8.16.3709.
The effect of ionizing radiation on DNA synthesis in control and ataxia telangiectasia (AT) lymphoblastoid cell lines was determined. A dose dependent decrease in DNA synthesis was observed in control cells, and the rate and extent of thi decrease in synthesis increased with time after irradiation. No decrease in DNA synthesis was obtained in AT cells, immediately following irradiation, at doses up to 400 rads. At longer times postirradiation, inhibition of synthesis increased but the extent of inhibition was less in AT cell than controls at all doses used. An immediate depression of DNA synthesis was evident in control cells after a radiation dose of 200 rads reaching a maximum at 90 min postirradiation. Little or no decrease in DNA synthesis was evident in AT cells up to 60 min after the same radiation dose, but a decrease occurred between 60 and 90 min after irradiation. The rate of recovery of DNA synthesis to normal levels was more rapid in AT cells than in controls.
测定了电离辐射对对照细胞和共济失调毛细血管扩张症（AT）淋巴母细胞系中DNA合成的影响。在对照细胞中观察到DNA合成呈剂量依赖性下降，且这种合成下降的速率和程度随照射后时间的增加而增加。在照射后立即给予高达400拉德的剂量时，AT细胞的DNA合成没有下降。在照射后较长时间，合成抑制增加，但在所有使用的剂量下，AT细胞的抑制程度均低于对照细胞。在给予200拉德辐射剂量后，对照细胞中DNA合成立即受到抑制，在照射后90分钟达到最大值。在相同辐射剂量后长达60分钟，AT细胞中DNA合成几乎没有下降，但在照射后60至90分钟之间出现了下降。AT细胞中DNA合成恢复到正常水平的速率比对照细胞更快。
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