Taylor K M, Devlin B J, Mittra S M, Gillan J G, Brannan J J, McKenna J M
Scand J Thorac Cardiovasc Surg. 1980;14(2):197-203. doi: 10.3109/14017438009100997.
A new experimental technique for the assessment of cerebral cellular damage during extracorporeal circulation is described. It is based upon the direct measurement of the enzyme creatine phosphokinase (CPK) and the brain-specific isoenzyme CPK-B in cerebrospinal fluid of dogs submitted to conventional techniques of cardiopulmonary bypass (CPB). Highly significant elevations occur during a 60 min period of CPB in CSF levels of total CPK and CPK-B isoenzyme. These elevated levels persist at 24 hours postoperation, despite full clinical recovery in the dogs. In a comparative study of the effects of introducing a 40 micrometer arterial line screen filter during the period of CPB, there was a highly significant reduction in total CPK and CPK-B levels in the filtered group (p < 0.005).
本文描述了一种用于评估体外循环期间脑细胞损伤的新实验技术。该技术基于对接受传统心肺转流术（CPB）的犬脑脊液中肌酸磷酸激酶（CPK）和脑特异性同工酶CPK-B的直接测量。在CPB 60分钟期间，脑脊液中总CPK和CPK-B同工酶水平出现高度显著升高。尽管犬在术后24小时临床完全恢复，但这些升高的水平仍然持续存在。在一项关于在CPB期间引入40微米动脉管路滤网的效果的对比研究中，过滤组的总CPK和CPK-B水平有高度显著降低（p < 0.005）。
