Hayden S A, Mills J W, Masland R M
Science. 1980 Oct;210(4468):435-7. doi: 10.1126/science.7433984.
The ganglion cell layer of the rabbit retina contains neurons that synthesize acetylcholine. To identify these neurons, the ganglion cells were labeled by retrograde transport of a fluorescent dye, and the acetylcholine-synthesizing cells of the same retinas were labeled by exposing the tissue to tritiated choline. Autoradiographs inspected by fluorescence microscopy showed that tritiated acetylcholine and the dye accumulated in different cells. Optic nerves of other animals were sectioned, causing degeneration of many neurons of the ganglion cell layer. This loss affected neither the retina's overall rate of acetylcholine synthesis nor the number of acetylcholine-containing cells in the ganglion cell layer. The acetylcholine-synthesizing neurons thus appear to be displaced amacrine cells.
兔视网膜的神经节细胞层包含合成乙酰胆碱的神经元。为了识别这些神经元，通过荧光染料的逆行运输标记神经节细胞，并且通过将组织暴露于氚化胆碱来标记同一视网膜中合成乙酰胆碱的细胞。通过荧光显微镜检查的放射自显影片显示，氚化乙酰胆碱和染料聚集在不同的细胞中。切断其他动物的视神经，导致神经节细胞层的许多神经元退化。这种损失既不影响视网膜乙酰胆碱合成的总体速率，也不影响神经节细胞层中含乙酰胆碱细胞的数量。因此，合成乙酰胆碱的神经元似乎是移位无长突细胞。
