Vaney D I, Young H M
National Vision Research Institute of Australia, Carlton, Victoria.
Brain Res. 1988 Jan 12;438(1-2):369-73. doi: 10.1016/0006-8993(88)91366-2.
In the ganglion cell layer of the rabbit retina, the inhibitory transmitter gamma-aminobutyric acid (GABA) and its analogues are accumulated by neurons that appear to match in size and number the population of displaced amacrine cells that synthesize the excitatory transmitter acetylcholine. In this double-label study, we have established directly that the cholinergic amacrine cells, selectively stained with diamidino-phenylindole, are strongly immunoreactive with GABA antisera. The coexistence of two classical transmitters, one excitatory and the other inhibitory, in this defined neuronal population, suggests that stimulation of the cholinergic amacrines may give rise to complex responses in their target neurons.
在兔视网膜的神经节细胞层中，抑制性递质γ-氨基丁酸（GABA）及其类似物被神经元积累，这些神经元在大小和数量上似乎与合成兴奋性递质乙酰胆碱的移位无长突细胞群体相匹配。在这项双标记研究中，我们直接确定，用双脒基苯基吲哚选择性染色的胆碱能无长突细胞与GABA抗血清有强烈的免疫反应。在这个特定的神经元群体中，两种经典递质（一种兴奋性递质和另一种抑制性递质）的共存表明，胆碱能无长突细胞的刺激可能会在其靶神经元中引发复杂的反应。
