El'bert L B, Gagarina A V, Khanina M K, Krutianskaia G L, Grachev V P
Vopr Virusol. 1980 May-Jun(3):341-5.
A concentrated and purified (lyophilized) preparation with a high immunological activity was obtained from formalin-inactivated tissue culture suspensions of tick-borne encephalitis (TBE) virus. Its high immunogenicity was demonstrated in mouse protection tests against fatal challenge with TBE and in the studies of antibody production in monkeys of two species. The preparation was shown to contain no infectious TBE virus, to be safe for small laboratory animals and monkeys. Trials of the preparation in 19 volunteers demonstrated it to be well tolerated and to induce virus-neutralizing antibody production in man. It also sensitized lymphoid cells when inoculated subcutaneously with Al2O3. These experimental materials indicate the possibility of developing a highly potent vaccine against tick-borne encephalitis by concentration and purification of TBE particle suspensions.
从蜱传脑炎（TBE）病毒的福尔马林灭活组织培养悬液中获得了一种具有高免疫活性的浓缩纯化（冻干）制剂。在针对TBE致死性攻击的小鼠保护试验以及对两种猴类抗体产生的研究中，证实了其高免疫原性。该制剂不含传染性TBE病毒，对小型实验动物和猴类是安全的。在19名志愿者身上进行的该制剂试验表明，它耐受性良好，能在人体内诱导产生病毒中和抗体。当与氧化铝皮下接种时，它还能使淋巴细胞致敏。这些实验材料表明，通过浓缩和纯化TBE颗粒悬液来开发一种高效的蜱传脑炎疫苗是有可能的。
