Iida M, Kobayashi H, Iizuka H
Z Allg Mikrobiol. 1980;20(7):449-57. doi: 10.1002/jobm.3630200704.
In an attempt to examine the specific metabolic relationship between the alkane substrates and lipid products the pattern of formation of cellular lipid, especially fatty acids, has been studied in the course of incubation of cells of C. rugosa with n-alkanes in mineral medium. C18:2, C18:1, C16:1 and C16:0 fatty acids were the major products formed, irrespective of the odd or even chain length of the alkane used as substrate. These unsaturated fatty acids reached a maximum in the stationary phase. In the case of cells grown on odd-chain n-alkanes (from C11 to C19), the proportion of odd-chain cellular fatty acids were markedly high, reaching 77--88% in the n-pentadecane and heptadecane-grown cells. The resulting acids are then metabolized by beta-oxidation or inserted directly or after elongation with C2-units into the cellular fatty acids. The total lipid content of n-alkane (n-C10-C20) grown cells in the stationary phase was 16.3--19.0% of th dry cell weight, which was about three times as much as that of glucose-grown cells. The chain length of alkane substrates had a significant effect on the lipid content. Ergosterol production from n-hexadecane was twice higher than that from glucose.
为了研究烷烃底物与脂质产物之间的特定代谢关系，在矿物质培养基中用正构烷烃培养皱纹盘鲍细胞的过程中，对细胞脂质尤其是脂肪酸的形成模式进行了研究。无论用作底物的烷烃链长是奇数还是偶数，C18:2、C18:1、C16:1和C16:0脂肪酸都是主要产物。这些不饱和脂肪酸在稳定期达到最大值。对于以奇数链正构烷烃（从C11到C19）生长的细胞，奇数链细胞脂肪酸的比例明显较高，在以正十五烷和正十七烷生长的细胞中达到77%-88%。生成的酸随后通过β-氧化进行代谢，或者直接插入细胞脂肪酸中，或者在与C2单元延长后插入细胞脂肪酸中。稳定期以正构烷烃（n-C10-C20）生长的细胞的总脂质含量为干细胞重量的16.3%-19.0%，约为以葡萄糖生长的细胞的三倍。烷烃底物的链长对脂质含量有显著影响。从正十六烷产生的麦角固醇比从葡萄糖产生的高出两倍。
