Murphy G L, Perry J J
J Bacteriol. 1983 Dec;156(3):1158-64. doi: 10.1128/jb.156.3.1158-1164.1983.
The cellular fatty acid composition of Mycobacterium vaccae JOB5 and Mycobacterium convolutum R22 was examined after growth on n-alkanes and compared with the fatty acids of the organisms after growth on 1-chlorohexadecane and 1-chlorooctadecane. Growth on n-alkanes resulted in normal fatty acid profiles. Mass spectral analyses indicated that, after growth on the terminally chlorinated n-alkanes, 75 to 86% of the fatty acids in M. convolutum and ca. 55% of the fatty acids in M. vaccae contained chlorine. Neither organism could utilize chloroacetate or 3-chloropropionate as sole source of carbon and energy. When these compounds were added to a growth medium with n-hexadecane as substrate, there was no evidence that chlorinated fatty acids were produced. Terminally chlorinated n-alkanes can be added to the list of n-alkanes, alkenes, and cyclohexylalkane derivatives that can be directly incorporated into cellular fatty acids of hydrocarbon-utilizing organisms.
在正构烷烃上生长后，检测了母牛分枝杆菌JOB5和卷曲分枝杆菌R22的细胞脂肪酸组成，并将其与在1-氯十六烷和1-氯十八烷上生长后的生物体脂肪酸进行了比较。在正构烷烃上生长产生了正常的脂肪酸谱。质谱分析表明，在末端氯化的正构烷烃上生长后，卷曲分枝杆菌中75%至86%的脂肪酸以及母牛分枝杆菌中约55%的脂肪酸含有氯。两种生物体都不能利用氯乙酸盐或3-氯丙酸盐作为唯一的碳源和能源。当将这些化合物添加到以正十六烷为底物的生长培养基中时，没有证据表明产生了氯化脂肪酸。末端氯化的正构烷烃可以添加到可直接掺入利用烃类生物体细胞脂肪酸中的正构烷烃、烯烃和环己基烷烃衍生物列表中。
