Caritis S N, Zeleznik A J
Am J Obstet Gynecol. 1980 Nov 15;138(6):677-80. doi: 10.1016/0002-9378(80)90087-3.
Human placental cells maintained were used to determine the direct effect of beta-adrenergic agonists on progesterone accumulation by the placenta. In five studies, when either of the beta-adrenergic receptor agonists, terbutaline or isoproterenol, 1.5 micrograms/ml, was incubated for 24 hours with the placental cells, significantly more progesterone was present in the culture medium than in the medium of matched controls. Compared with the medium of matched controls, progesterone in medium increased 36 +/- 6% (mean +/- SE) when cells were treated with terbutaline and 38 +/- 7% when cells were treated with isoproterenol. In two dose-response studies with terbutaline, a minimal dose of 0.15 micrograms/ml was required to significantly increase progesterone in medium compared with that in matched controls. In three studies, propranolol at a concentration of 1.5 micrograms/ml completely blocked the stimulatory effect of 1.5 micrograms/ml of terbutaline.
使用人胎盘细胞来确定β-肾上腺素能激动剂对胎盘孕酮积累的直接影响。在五项研究中，当β-肾上腺素能受体激动剂特布他林或异丙肾上腺素中的任何一种以1.5微克/毫升的浓度与胎盘细胞一起孵育24小时时，培养基中存在的孕酮明显多于匹配对照的培养基。与匹配对照的培养基相比，用特布他林处理细胞时，培养基中的孕酮增加了36±6%（平均值±标准误），用异丙肾上腺素处理细胞时增加了38±7%。在两项关于特布他林的剂量反应研究中，与匹配对照相比，需要0.15微克/毫升的最小剂量才能显著增加培养基中的孕酮。在三项研究中，浓度为1.5微克/毫升的普萘洛尔完全阻断了1.5微克/毫升特布他林的刺激作用。
