Arko R J, Wong K H
Am J Vet Res. 1980 Jul;41(7):989-93.
An infection model in laboratory mice for studying the bacterium (proposed name Haemophilus equigenitalis) causing contagious equine metritis is described. Small porous chambers were implanted subcutaneously into mice and after 1 to 3 weeks were inoculated with H equigenitalis. Infections that persisted for > 30 days were established by direct transfer of infective chamber fluid or by injection of laboratory-grown cultures. Immunization of mice with formaldehyde-treated cells induced significant, strain-related immunity to infection and did not appear to require complement as a protection mediator. Substantial differences in staining characteristics and cell morphology were observed between bacteria grown in mouse chambers and those grown on a laboratory medium. These differences were more apparent in smears of the organism stained by a modified Gimenez method than in smears stained by the conventional Gram stain. This murine model may be a convenient and inexpensive method for studying the immunobiology of this newly discovered animal pathogen.
描述了一种用于研究引起马传染性子宫炎的细菌（暂定名马生殖嗜血杆菌）的实验室小鼠感染模型。将小型多孔室皮下植入小鼠体内，1至3周后接种马生殖嗜血杆菌。通过直接转移感染性腔室液体或注射实验室培养的培养物建立持续超过30天的感染。用甲醛处理的细胞免疫小鼠可诱导出显著的、与菌株相关的抗感染免疫力，并且似乎不需要补体作为保护介质。在小鼠腔室中生长的细菌与在实验室培养基上生长的细菌之间，观察到染色特征和细胞形态存在显著差异。与传统革兰氏染色的涂片相比，用改良吉姆enez方法染色的生物体涂片上这些差异更明显。这种小鼠模型可能是研究这种新发现的动物病原体免疫生物学的一种方便且廉价的方法。
