Swaney L M, Breese S S
Am J Vet Res. 1980 Jan;41(1):127-32.
Haemophilus equigenitalis, a proposed new species of Haemophilus and the causative agent of contagious equine metritis, a venereal disease of the horse, had ultrastructural characteristics of gram-negative bacteria. The organism additionally had a small, threadlike capsule that was removed by heating in phosphate-buffered saline solution. Heating also detached the outer membrane from the cytoplasmic membrane. The capsule could only be demonstrated when bacterial were stained with ruthenium red during the preparation of ultrathin sections. The gross morphology of newly isolated organisms (rodlike or coccal) depended upon the medium on which they were grown.
马生殖嗜血杆菌是嗜血杆菌属中一个拟议的新种,是马传染性子宫炎(一种马的性病)的病原体,具有革兰氏阴性菌的超微结构特征。该微生物还具有一个小的丝状荚膜,可通过在磷酸盐缓冲盐溶液中加热去除。加热还会使外膜与细胞质膜分离。只有在制备超薄切片时用钌红对细菌进行染色,才能显示出荚膜。新分离出的微生物的总体形态(杆状或球状)取决于它们生长的培养基。