Wendelberger-Schieweg G, Hüttermann A, Haugli F B
Arch Microbiol. 1980 Jun;126(2):109-15. doi: 10.1007/BF00511215.
The specificity action of cycloheximide was tested using a cycloheximide resistant mutant of Physarum polycephalum. This resistance has previously been shown to reside with the ribosomes, making cytoplasmic protein synthesis refractile to the action of the drug. We show here that cycloheximide in the mutant strain causes specific alterations in metabolism without influencing the growth rate. These are: 1. lowered specific activity of glutamate dehydrogenase during starvation, 2. alteration of the molecular weight of glutamate dehydrogenase, 3. inhibition of uptake of amino acids from the medium into the internal pools. Possible explanations for these effects of cycloheximide outside of protein synthesis per se are considered. We conclude that cycloheximide may not be considered a specific inhibitor of protein synthesis, and that a causal relationship between protein synthesis and any biological process cannot be claimed unless such specificity is demonstrated in each case, preferably by use of mutants.
使用多头绒泡菌的环己酰亚胺抗性突变体测试了环己酰亚胺的特异性作用。此前已证明这种抗性存在于核糖体中，使得细胞质蛋白质合成对该药物的作用具有抗性。我们在此表明，突变菌株中的环己酰亚胺会导致代谢发生特异性改变，而不影响生长速率。这些改变包括：1. 饥饿期间谷氨酸脱氢酶的比活性降低；2. 谷氨酸脱氢酶分子量改变；3. 抑制氨基酸从培养基摄取到内部池。本文考虑了环己酰亚胺在蛋白质合成本身之外产生这些效应的可能解释。我们得出结论，环己酰亚胺可能不能被视为蛋白质合成的特异性抑制剂，并且除非在每种情况下都证明了这种特异性，最好是使用突变体证明，否则不能声称蛋白质合成与任何生物学过程之间存在因果关系。
