多头绒泡菌中的DNA复制：在对放线菌酮敏感和耐药的菌株中，在放线菌酮存在的情况下体内合成的DNA复制产物的特征。
DNA replication in Physarum polycephalum: characterization of DNA replication products made in vivo in the presence of cycloheximide in strains sensitive and resistant to cycloheximide.
作者信息
Funderud S, Haugli F
出版信息
Nucleic Acids Res. 1977 Feb;4(2):405-13. doi: 10.1093/nar/4.2.405.
Abstract
Synchronous plasmodia of cycloheximide-sensitive and cycloheximide-resistant strains of Physarum polycephalum were labelled with 3[H]-deoxyadenosine in pulse and pulse-chase experiments in presence and absence of cycloheximide. The replication products were studied with alkaline sucrose gradient sedimentation analysis. We show that the action of cycloheximide on DNA replication in Physarum is mediated through the ribosome, since the ribosomally located resistance also makes the plasmodial DNA replication refractile to the action of cycloheximide. Cycloheximide caused inhibition of three stages in DNA replication in the wild type: first, the formation of primary replication units ("Okazaki" size fragments), secondly, the ligation of primary units into secondary ("Replicon" size) units and thirdly, the ligation of secondary units into mature DNA.
摘要
在有无放线菌酮的情况下，通过脉冲和脉冲追踪实验，用³[H] - 脱氧腺苷对多头绒泡菌的放线菌酮敏感型和放线菌酮抗性菌株的同步原质团进行标记。利用碱性蔗糖梯度沉降分析法研究复制产物。我们发现，放线菌酮对多头绒泡菌DNA复制的作用是通过核糖体介导的，因为核糖体定位的抗性也使原质团DNA复制对放线菌酮的作用具有抗性。放线菌酮对野生型DNA复制的三个阶段产生抑制作用：第一，初级复制单元（“冈崎”大小片段）的形成；第二，初级单元连接成次级（“复制子”大小）单元；第三，次级单元连接成成熟DNA。
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