多头绒泡菌中的DNA复制：在对放线菌酮敏感和耐药的菌株中，在放线菌酮存在的情况下体内合成的DNA复制产物的特征。
DNA replication in Physarum polycephalum: characterization of DNA replication products made in vivo in the presence of cycloheximide in strains sensitive and resistant to cycloheximide.
作者信息
Funderud S, Haugli F
出版信息
Nucleic Acids Res. 1977 Feb;4(2):405-13. doi: 10.1093/nar/4.2.405.
Abstract
Synchronous plasmodia of cycloheximide-sensitive and cycloheximide-resistant strains of Physarum polycephalum were labelled with 3[H]-deoxyadenosine in pulse and pulse-chase experiments in presence and absence of cycloheximide. The replication products were studied with alkaline sucrose gradient sedimentation analysis. We show that the action of cycloheximide on DNA replication in Physarum is mediated through the ribosome, since the ribosomally located resistance also makes the plasmodial DNA replication refractile to the action of cycloheximide. Cycloheximide caused inhibition of three stages in DNA replication in the wild type: first, the formation of primary replication units ("Okazaki" size fragments), secondly, the ligation of primary units into secondary ("Replicon" size) units and thirdly, the ligation of secondary units into mature DNA.
摘要
在有无放线菌酮的情况下，通过脉冲和脉冲追踪实验，用³[H] - 脱氧腺苷对多头绒泡菌的放线菌酮敏感型和放线菌酮抗性菌株的同步原质团进行标记。利用碱性蔗糖梯度沉降分析法研究复制产物。我们发现，放线菌酮对多头绒泡菌DNA复制的作用是通过核糖体介导的，因为核糖体定位的抗性也使原质团DNA复制对放线菌酮的作用具有抗性。放线菌酮对野生型DNA复制的三个阶段产生抑制作用：第一，初级复制单元（“冈崎”大小片段）的形成；第二，初级单元连接成次级（“复制子”大小）单元；第三，次级单元连接成成熟DNA。
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DNA replication in Physarum polycephalum: characterization of DNA replication products made in vivo in the presence of cycloheximide in strains sensitive and resistant to cycloheximide.多头绒泡菌中的DNA复制：在对放线菌酮敏感和耐药的菌株中，在放线菌酮存在的情况下体内合成的DNA复制产物的特征。
Nucleic Acids Res. 1977 Feb;4(2):405-13. doi: 10.1093/nar/4.2.405.
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DNA replication in Physarum polycephalum.多头绒泡菌中的DNA复制。
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引用本文的文献
1
Muliple sites of action of cycloheximide in addition to inhibition of protein synthesis in Physarum polycephalum.除了抑制多头绒泡菌中的蛋白质合成外，放线菌酮的多个作用位点。
Arch Microbiol. 1980 Jun;126(2):109-15. doi: 10.1007/BF00511215.
2
Cycloheximide resistance of Physarum polycephalum.多头绒泡菌对放线菌酮的抗性
J Bacteriol. 1980 Aug;143(2):897-905. doi: 10.1128/jb.143.2.897-905.1980.
3
In the higher plant Pisum sativum maturation of nascent DNA is blocked by cycloheximide, but only after 4-8 replicons are joined.在高等植物豌豆中，新生DNA的成熟被环己酰亚胺阻断，但仅在4-8个复制子连接之后。
Nucleic Acids Res. 1982 Oct 11;10(19):6191-205. doi: 10.1093/nar/10.19.6191.
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DNA replication in Physarum polycephalum: electron microscopic analysis of patterns of DNA replication in the presence of cycloheximide.多头绒泡菌中的DNA复制：在放线菌酮存在下DNA复制模式的电子显微镜分析
J Cell Biol. 1982 Oct;95(1):323-31. doi: 10.1083/jcb.95.1.323.
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DNA replication in Physarum polycephalum: bidirectional replication of DNA within replicons.多头绒泡菌中的DNA复制：复制子内DNA的双向复制。
Nucleic Acids Res. 1978 Mar;5(3):713-21. doi: 10.1093/nar/5.3.713.
6
Preferential binding of S-phase proteins to temporally-characteristic units of replication in Physarum polycephalum.多头绒泡菌中S期蛋白与复制时间特征单元的优先结合。
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DNA replication in Physarum polycephalum: electron microscopic and autoradiographic analysis of replicating DNA from defined stages of the S-period.多头绒泡菌中的DNA复制：对S期特定阶段复制DNA的电子显微镜和放射自显影分析
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Exp Cell Res. 1973 Jul;80(1):15-26. doi: 10.1016/0014-4827(73)90270-x.
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The effect of cycloheximide on the synthesis of major and satellite DNA components in Physarum polycephalum.放线菌酮对多头绒泡菌中主要和卫星DNA成分合成的影响。
Biochim Biophys Acta. 1972 Dec 6;287(2):232-5. doi: 10.1016/0005-2787(72)90372-3.
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Genetics and biochemistry of cycloheximide resistance in Physarum polycephalum.多头绒泡菌中放线菌酮抗性的遗传学与生物化学
Mol Gen Genet. 1972;118(2):97-107. doi: 10.1007/BF00267081.
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Fine control of DNA synthesis in developing chick red blood cells.发育中的鸡红细胞中DNA合成的精细调控。
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Limited DNA synthesis in the absence of protein synthesis in Physarum polycephalum.多头绒泡菌在缺乏蛋白质合成的情况下DNA合成受限。
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Deficiency of joining of Okazaki-type fragments in absence of cellular protein synthesis.在缺乏细胞蛋白质合成的情况下冈崎型片段连接缺陷。
Eur J Biochem. 1975 Jun;54(2):351-8. doi: 10.1111/j.1432-1033.1975.tb04145.x.
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