Ward C W, Dopheide T A, Inglis A S
Aust J Biol Sci. 1980 May;33(2):137-51. doi: 10.1071/bi9800137.
The amino acid sequence of cyanogen bromide peptide CN2 from the heavy chain (HA1) of the haemagglutinin of the Hong Kong variant A/Memphis/102/72 has been obtained by direct, automated sequence analysis on the whole fragment and by manual dansyl-Edman degradation of tryptic, peptic and chymotryptic peptides. It was found to contain 92 amino acid residues, including a large, insoluble, tryptic core peptide (residues 62-87). It did not contain any half-cystine residues or carbohydrate. The determination of its structure was complicated by the presence of an Asn-Ile bond at positions 48-49 which was readily cleaved by both trypsin and chymotrypsin.
通过对香港变种A/孟菲斯/102/72血凝素重链（HA1）的溴化氰肽CN2整个片段进行直接自动序列分析，以及对胰蛋白酶、胃蛋白酶和胰凝乳蛋白酶肽段进行丹磺酰-埃德曼手动降解，已获得其氨基酸序列。发现它含有92个氨基酸残基，包括一个大的不溶性胰蛋白酶核心肽段（第62 - 87位残基）。它不含任何半胱氨酸残基或碳水化合物。其结构的确定因第48 - 49位存在一个天冬酰胺-异亮氨酸键而变得复杂，该键很容易被胰蛋白酶和胰凝乳蛋白酶切割。
