Dopheide T A, Ward C W
Eur J Biochem. 1978 Apr 17;85(2):393-8. doi: 10.1111/j.1432-1033.1978.tb12251.x.
The reduced carboxymethylated heavy chain (HA1) of a Hong Kong influenza haemagglutinin (H3) was digested with cyanogen bromide to give five peptides. The sequences of three of these peptides, together with the sequences of two methionine overlap peptides were combined to give the C-terminal 68-residue sequence of the heavy chain. Threonine is the C-terminal residue of the heavy chain, an unexpected result in view of the generation of the heavy and light chains by the proteolytic cleavage of a pro-haemagglutinin during virus assembly. Three half-cystine residues occur close together at positions 24, 48 and 52 residues in from the C-terminus. A carbohydrate group is attached to an asparagine residue 44 residues from the C-terminus; it contains 5 residues mannose and 2 residues glucosamine.
香港流感血凝素（H3）的还原羧甲基化重链（HA1）用溴化氰消化，得到五个肽段。将其中三个肽段的序列，与两个甲硫氨酸重叠肽段的序列合并，得到重链C端的68个残基序列。苏氨酸是重链的C端残基，鉴于病毒组装过程中前血凝素经蛋白水解裂解产生重链和轻链，这是一个意外的结果。三个半胱氨酸残基在距C端24、48和52个残基的位置紧密相邻。一个糖基连接在距C端44个残基的天冬酰胺残基上；它含有5个甘露糖残基和2个葡糖胺残基。
