Yamada A, Okuno Y, Takahashi M, Nakajima K
Biken J. 1980 Mar;23(1):25-32.
A live attenuated influenza A (H3N2) strain "KO-1" was developed by recombination of wild influenza A/Kumamoto/22/76 (H3N2) with attenuated A/Okuda/57 (H2N2) followed by passage in developing chick eggs in the presence of horse serum inhibitor. The virus strain "KO-1" obtained is inhibitor-resistant, and has hemagglutinating (HA) and neuraminidase (NA) antigenicity derived from the wild parent virus. RNA analysis revealed that one RNA segment (corresponding to M protein) was derived from the A/Okuda/57 strain. The "KO-1" strain shows restricted growth in the lungs of Syrian hamsters as compared with that of the parent A/Kumamoto/76 strain and no transmission of virus from the hamsters infected with "KO-1" strain by inhalation ot untreated hamsters housed in the same cage was detected serologically. In contrast, the parent wild A/Kumamoto/76 strain induced contact infection under the same condition. The attenuated "KO-1" strain was administered by inhalation to a few children of 3 to 4 years old in a preliminary clinical trial. A good antibody response was observed with no clinical reaction.
一种甲型流感病毒（H3N2）减毒株“KO - 1”是通过将野生甲型流感病毒/熊本/22/76（H3N2）与减毒的甲型流感病毒/奥田/57（H2N2）重组，随后在马血清抑制剂存在的情况下于发育中的鸡胚中传代而培育出来的。所获得的病毒株“KO - 1”对抑制剂具有抗性，并且具有源自野生亲本病毒的血凝素（HA）和神经氨酸酶（NA）抗原性。RNA分析表明，一个RNA片段（对应于M蛋白）源自甲型流感病毒/奥田/57株。与亲本甲型流感病毒/熊本/76株相比，“KO - 1”株在叙利亚仓鼠肺中的生长受到限制，并且血清学检测未发现通过吸入感染“KO - 1”株的仓鼠将病毒传播给同笼饲养的未处理仓鼠。相比之下，亲本野生甲型流感病毒/熊本/76株在相同条件下可引发接触感染。在一项初步临床试验中，通过吸入方式给几名3至4岁的儿童接种了减毒的“KO - 1”株。观察到良好的抗体反应，且无临床反应。
