Soliman K M, Bernardin J E, Qualset C O
Biochem Genet. 1980 Jun;18(5-6):465-82. doi: 10.1007/BF00484395.
An Agropyron chromosome having a gene conferring blue color on the aleurone layer of the kernel endosperm causes a 15% increase in total grain protein content when it is added to the common wheat (2n=42) complement. In contrast, there is no effect of this chromosome on total protein content if it replaced part of a wheat chromosome. Endosperm protein components of isolines having blue aleurone due to the Agropyron chromosomes being added (2n=44) or translocated (2n=42) were compared to normal nonblue isoline counterparts. Gliadin proteins separated by aluminum lactate (pH 3.2) polyacrylamide gel electrophoresis (PAGE) in one or two dimensions showed greater staining intensity for the blue addition isolines (2n=44) than nonblue (2n=42) isolines. However the 42-chromosome blue isoline did not show increased protein staining over the nonblue isoline, but at least five protein differences were detected between the lines. SDS-PAGE showed that blue and nonblue differences were expressed primarily in the gliadins, but also in the glutenin, globulin, and albumin proteins.
一条具有使籽粒胚乳糊粉层呈现蓝色基因的冰草染色体，当它添加到普通小麦（2n = 42）基因组中时，会使籽粒总蛋白含量增加15%。相比之下，如果该染色体取代了部分小麦染色体，则对总蛋白含量没有影响。将因添加（2n = 44）或易位（2n = 42）冰草染色体而具有蓝色糊粉层的近等基因系的胚乳蛋白组分，与正常的非蓝色近等基因系对应物进行了比较。通过乳酸铝（pH 3.2）聚丙烯酰胺凝胶电泳（PAGE）在一维或二维条件下分离的醇溶蛋白，对于添加蓝色染色体的近等基因系（2n = 44），其染色强度比非蓝色近等基因系（2n = 42）更强。然而，42条染色体的蓝色近等基因系在蛋白染色方面并未比非蓝色近等基因系增加，但在这两个品系之间至少检测到了五个蛋白差异。SDS - PAGE显示，蓝色和非蓝色的差异主要表现在醇溶蛋白中，但在谷蛋白、球蛋白和清蛋白中也有体现。
