Payne P I, Holt L M, Reader S M, Miller T E
Biochem Genet. 1987 Feb;25(1-2):53-65. doi: 10.1007/BF00498951.
The proteins of Hordeum chilense grain were resolved into 25 major components by two-dimensional electrophoresis. Their solubilities in aqueous alcohol solutions were determined to distinguish prolamin storage proteins from metabolic and structural proteins. The prolamins were divided into two groups, based on the presence or absence of intermolecular disulfide bonds determined by gel-filtration chromatography. Using an incomplete set of Chinese Spring wheat-H. chilense disomic addition lines, the structural genes of 21 of the 26 most dominant seed proteins were assigned to chromosomes. The great majority of the prolamin genes, including those coding for a high molecular weight (HMW) prolamin subunit, was present on chromosome 1 Hch. However, a small number of prolamin genes also occurred on chromosomes 5 Hch and 7 Hch. A minor protein, probably belonging to the nonstorage group of proteins, is coded by genes on 5 Hch. Various ditelosomic addition lines and ditelosomic and disomic substitution lines for chromosome 7 Hch were also analyzed by electrophoresis. This technique revealed that the genes for three major prolamins occur on the beta arm of chromosome 7 Hch and that a gene for a minor protein, also thought to be a prolamin, occurs on the alpha arm. These results are discussed in relation to the evolution of prolamin genes in the Triticeae.
通过二维电泳将智利大麦籽粒蛋白分离为25个主要组分。测定它们在乙醇水溶液中的溶解度，以区分醇溶谷蛋白储存蛋白与代谢蛋白和结构蛋白。根据凝胶过滤色谱法测定的分子间二硫键的有无，将醇溶谷蛋白分为两组。利用一套不完整的中国春小麦-智利大麦二体附加系，将26种最主要种子蛋白中的21种的结构基因定位到染色体上。绝大多数醇溶谷蛋白基因，包括那些编码高分子量（HMW）醇溶谷蛋白亚基的基因，位于1Hch染色体上。然而，少数醇溶谷蛋白基因也出现在5Hch和7Hch染色体上。一种可能属于非储存蛋白组的次要蛋白，由5Hch染色体上的基因编码。还通过电泳分析了7Hch染色体的各种端体附加系以及端体和二体代换系。该技术表明，三种主要醇溶谷蛋白的基因位于7Hch染色体的β臂上，而一种也被认为是醇溶谷蛋白的次要蛋白的基因位于α臂上。结合小麦族醇溶谷蛋白基因的进化对这些结果进行了讨论。
