Raziuddin S
Biochim Biophys Acta. 1980 Nov 7;620(2):193-204. doi: 10.1016/0005-2760(80)90201-5.
Lipopolysaccharides from Vibrio cholerae, NIH 41, Ogawa 5321 and Inaba 66/64 were treated with succinic anhydride, phthalic anhydride and dinitrophenyl ethylene diamine, and the resultant derivatives, sodium succinyl lipopolysaccharide, sodium phthalyl lipopolysaccharide, and dinitrohpenyl lipopolysaccharide obtained respectively were investigated for various biological activities. The succinylation and phthalylation of lipopolysaccharide decreased the 3-hydroxy lauric acid, a major ester-linked fatty acid of these bacteria, and as a result of which these modified products exhibited lower toxic activities in chick embryos, mouse and in generalized local Shwartzmann reaction in rabbits than their parent lipopolysaccharides. The dinitrophenylation of lipopolysaccharide increased its toxicity in chick embryos and mice, but dinitrophenyl lipopolysaccharide was completely inactive in Shwartzmann reaction in rabbits. However, despite the loss of these biological activities, these modified derivatives of lipopolysaccharide retained and increased the activities in pyrogenecity and in various immunological properties.
用琥珀酸酐、邻苯二甲酸酐和二硝基苯乙二胺处理来自霍乱弧菌NIH 41、小川5321和稻叶66/64的脂多糖，并分别研究所得衍生物琥珀酰脂多糖钠、邻苯二甲酰脂多糖钠和二硝基苯脂多糖的各种生物学活性。脂多糖的琥珀酰化和邻苯二甲酰化降低了这些细菌的主要酯键连接脂肪酸3-羟基月桂酸的含量，结果这些修饰产物在鸡胚、小鼠和兔的全身性局部施瓦茨曼反应中表现出比其亲本脂多糖更低的毒性活性。脂多糖的二硝基苯化增加了其在鸡胚和小鼠中的毒性，但二硝基苯脂多糖在兔的施瓦茨曼反应中完全无活性。然而，尽管失去了这些生物学活性，这些脂多糖的修饰衍生物仍保留并增强了热原性和各种免疫学特性方面的活性。
