小川型和稻叶型霍乱弧菌脂多糖中5-磷酸-2-酮基脱氧辛酸的存在情况。
Occurrence of 2-keto-deoxyoctonic acid 5-phosphate in lipopolysaccharides of Vibrio cholerae Ogawa and Inaba.
作者信息
Brade H
出版信息
J Bacteriol. 1985 Feb;161(2):795-8. doi: 10.1128/jb.161.2.795-798.1985.
Abstract
A phosphorylated 2-keto-3-deoxyoctonic acid (KDO) was released from the lipopolysaccharides of Vibrio cholerae Ogawa and Inaba after strong acid hydrolysis. The phosphorylated KDO was identified by gas-liquid chromatography and mass spectrometry after reduction and permethylation as KDO-5-phosphate and an isomer of it being phosphorylated at position 7 or 8. After treatment with alkaline phosphatase, KDO was detected by gas-liquid chromatography and mass spectrometry. It was indistinguishable from authentic 2-keto-3-deoxy-D-manno-octonic acid.
摘要
在强酸水解后，磷酸化的2-酮-3-脱氧辛酸（KDO）从霍乱弧菌小川型和稻叶型的脂多糖中释放出来。经还原和全甲基化后，通过气液色谱和质谱鉴定该磷酸化的KDO为5-磷酸-KDO及其在7位或8位磷酸化的异构体。用碱性磷酸酶处理后，通过气液色谱和质谱检测到KDO。它与 authentic 2-酮-3-脱氧-D-甘露辛酮酸无法区分。 （注：这里“authentic”可译为“真实的、正宗的、标准的”等，具体意思需结合上下文确定，暂保留英文未译，因为不确定在此处最合适的中文表述）
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