Stafford C J, Reichel W L, Swineford D M, Prouty R M, Gay M L
J Assoc Off Anal Chem. 1978 Jan;61(1):8-14.
A procedure is described for determining Kepone (decachlorooctahydro-1,3,4-metheno-2H-cyclobuta [cd] pentalene-2-one) residues in avian egg, liver, and tissue. Samples were extracted with benzene-isopropanol, and the extract was cleaned up with fuming H2SO4-concentrated H2SO4. Kepone was separated from organochlorine pesticides and polychlorinated biphenyls on a Florisil column and analyzed by electron capture gas-liquid chromatography (GLC). The average recovery from spiked tissues was 86%. The analyses performed on 14 bald eagle carcasses and livers, 3 bald eagle eggs, and 14 osprey eggs show measurable levels which indicate that Kepone accumulates in the tissues of fish-eating birds. Residues were confirmed by GLC-mass spectrometry.
描述了一种测定禽蛋、肝脏和组织中开蓬（十氯八氢-1,3,4-亚甲基-2H-环丁烷并[cd]戊搭烯-2-酮）残留量的方法。样品用苯 - 异丙醇提取，提取物用发烟硫酸 - 浓硫酸净化。开蓬在弗罗里硅土柱上与有机氯农药和多氯联苯分离，并通过电子捕获气液色谱法（GLC）进行分析。加标组织的平均回收率为86%。对14只白头鹰尸体和肝脏、3枚白头鹰蛋以及14枚鹗蛋进行的分析显示出可测量的含量，这表明开蓬在食鱼鸟类的组织中积累。残留量通过GLC - 质谱法得到确认。
