Moseman R F, Crist H L, Edgerton T R, Ward M K
Arch Environ Contam Toxicol. 1977;6(2-3):221-31. doi: 10.1007/BF02097763.
The pollution of the environment with Kepone (decachlorooctahydro-1,3,4-metheno-2H-cyclobuta[cd]pentalen-2-one) by an industrial manufacturer of the pesticide resulted in the contamination of several terrestrial media, including biological life, near the entry of the insecticide into the ecosystem. The substrata investigated and for which residue methodology was developed included river sediment, soil, water, shellfish, and finfish. Rigorous extraction techniques utilizing the Soxhlet apparatus and the Polytron tissue homogenizer were required for complete removal of Kepone from the samples. Finfish tissue was the most difficult to analyze. For this type of substratum, a preliminary cleanup by gel permeation chromatography was required to remove most of the lipid material followed by a micro Florisil column elution to eliminate polychlorinated biphenyls (PCB'S). Cleanup of shellfish and other environmental samples was accomplished with a micro Florisil column only. Electron capture gas chromatography was used to analyze the sample extracts. Recoveries of Kepone from fortified samples averaged 84% or greater.
一家杀虫剂工业制造商用开蓬（十氯八氢-1,3,4-亚甲基-2H-环丁烷[cd]戊烯-2-酮）对环境造成污染，导致在该杀虫剂进入生态系统的入口附近，包括生物在内的几种陆地介质受到污染。所研究的且已开发出残留量测定方法的基质包括河流沉积物、土壤、水、贝类和有鳍鱼类。为了从样品中完全去除开蓬，需要使用索氏提取器和保利tron组织匀浆器的严格提取技术。有鳍鱼类组织最难分析。对于这类基质，需要先通过凝胶渗透色谱法进行初步净化，以去除大部分脂质物质，然后用微型弗罗里硅土柱洗脱以消除多氯联苯（PCBs）。贝类和其他环境样品仅用微型弗罗里硅土柱进行净化。用电子捕获气相色谱法分析样品提取物。加标样品中开蓬的回收率平均为84%或更高。
