多形核白细胞对乳胶的吞噬作用：唾液酸基团的作用。

Latex phagocytosis by polymorphonuclear leukocytes: role of sialic acid groups.

作者信息

Beukers H, Deierkauf F A, Blom C P, Deierkauf M, Scheffers C C, Riemersma J C

出版信息

Chem Biol Interact. 1980 Dec;33(1):91-100. doi: 10.1016/0009-2797(80)90046-0.

Abstract

Polystyrene latex particles are rapidly phagocytized by rabbit polymorphonuclear (PMN) leukocytes. The uptake is influenced by macromolecules which have the effect of altering the surface charge of the latex particle. The influence of polylysines of varying chain length on the surface charge of latex particles and of PMN cells was studied by micro-electrophoresis. Charge reversal at the latex surface was found to occur at concentrations considerably below that at which the surface charge of the PMN cells is reversed. Phagocytosis of latex by PMN cells is enhanced in the presence of low concentrations of long-chain polylysines. The enhancement of phagocytosis is strongly reduced if PMN cells are treated with neuraminidase. This suggests participation of sialic acid groups in a stage of particle-cell interaction which precedes engulfment.

摘要

聚苯乙烯乳胶颗粒能被兔多形核（PMN）白细胞迅速吞噬。摄取过程受大分子影响，这些大分子会改变乳胶颗粒的表面电荷。通过微电泳研究了不同链长的聚赖氨酸对乳胶颗粒和PMN细胞表面电荷的影响。发现乳胶表面电荷反转时的浓度远低于PMN细胞表面电荷反转时的浓度。在低浓度长链聚赖氨酸存在的情况下，PMN细胞对乳胶的吞噬作用增强。如果用神经氨酸酶处理PMN细胞，吞噬作用的增强会大大降低。这表明唾液酸基团参与了吞噬前颗粒与细胞相互作用的一个阶段。