Ishikawa Y, Muramatsu N, Ohshima H, Kondo T
Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
J Biomater Sci Polym Ed. 1991;2(1):53-60. doi: 10.1163/156856291x00052.
The effect of the size of foreign particles on phagocytosis by guinea-pig polymorphonuclear leucocytes (PMNs) was investigated using latex particles having approximately the same surface potentials but different sizes. Two types of latexes with different negative surface potentials, those having amino groups (Ami-latexes) and those having carboxylic acid groups (Ca-latexes), were used in this experiment. Since Ca-latexes had different surface potentials for different particle sizes, the carboxylic acid groups were modified by the carbodiimide method to make the surface potentials almost equal. Phagocytosis was estimated by the rate of oxygen consumption. It was found that the phagocytosis reached its maximum when the latex particle size was about 0.5 microns. This finding was plausibly explained by considering the two probabilities that a latex particle comes into contact with a PMN surpassing a potential barrier and the contact takes place at certain receptor areas favourable to phagocytosis on the PMN surface.
使用表面电位大致相同但大小不同的乳胶颗粒，研究了外来颗粒大小对豚鼠多形核白细胞（PMN）吞噬作用的影响。本实验使用了两种具有不同负表面电位的乳胶，即具有氨基的乳胶（氨基乳胶）和具有羧酸基团的乳胶（钙乳胶）。由于钙乳胶对于不同粒径具有不同的表面电位，因此通过碳二亚胺法对羧酸基团进行修饰，以使表面电位几乎相等。通过耗氧率来估计吞噬作用。结果发现，当乳胶颗粒大小约为0.5微米时，吞噬作用达到最大值。通过考虑乳胶颗粒与PMN接触超过势垒以及接触发生在PMN表面有利于吞噬作用的特定受体区域这两种可能性，可以合理地解释这一发现。
