采用放射免疫分析法测定肠道抗体。
Measurement of intestinal antibody by radioimmunoassay.
作者信息
La Brooy J T, Rowley D, Shearman D J
出版信息
Clin Exp Immunol. 1980 Aug;41(2):281-9.
The study of antibody responses in the intestine has been greatly hampered by lack of reproducible sensitive assays. An assay for measuring antibody against bacteria capable of regularly detecting gut antibody in gastroenteritis is described. It is based on absorption of antibody onto bacteria and measurement of the amount of antibody bound using radiolabelled anti-immunoglobulin antibody. Anti-light chain antibody is used to detect all classes of antibody as well as partially degraded antibody which retains the capacity to bind; anti-alpha and anti-gamma antibody is used to measure IgA and IgG antibody. The sensitivity of the assay depends on the use of anti-immunoglobulin antibody purified by affinity chromatography and allows measurement of nanogram amounts of antibody. Its specificity and kinetics are described and the particular advantages it provides in the measurement of antibacterial antibody in the intestine are discussed.
由于缺乏可重复的灵敏检测方法，肠道抗体反应的研究受到了极大阻碍。本文描述了一种用于测量针对细菌的抗体的检测方法，该方法能够在肠胃炎中定期检测肠道抗体。它基于抗体与细菌的结合以及使用放射性标记的抗免疫球蛋白抗体测量结合的抗体量。抗轻链抗体用于检测所有类别的抗体以及保留结合能力的部分降解抗体；抗α和抗γ抗体用于测量IgA和IgG抗体。该检测方法的灵敏度取决于使用通过亲和色谱纯化的抗免疫球蛋白抗体，能够测量纳克量的抗体。文中描述了其特异性和动力学，并讨论了它在测量肠道抗菌抗体方面所具有的特殊优势。
Zotero 插件