哺乳动物中期染色体对核酸内切酶消化的反应。
Responses of mammalian metaphase chromosomes to endonuclease digestion.
作者信息
Sahasrabuddhe C G, Pathak S, Hsu T C
出版信息
Chromosoma. 1978 Dec 6;69(3):331-8. doi: 10.1007/BF00332136.
PMID:743903
Abstract
Digestion of fixed metaphase chromosomes by endonucleases (micrococcal nuclease and DNase II) under optimal digestion conditions followed by Giemsa staining produces sharp banding patterns identical to G-bands. In 3H-thymidine labeled, synchronized metaphase cells of the chinese hamster (CHO line), the band induction is accompanied by the removal of DNA. The single strand specific nuclease S1 and DNase I do not produce such banding patterns.
摘要
在最佳消化条件下，用核酸内切酶（微球菌核酸酶和脱氧核糖核酸酶II）消化固定的中期染色体，随后进行吉姆萨染色，可产生与G带相同的清晰条带模式。在3H-胸腺嘧啶核苷标记的、同步化的中国仓鼠中期细胞（CHO系）中，条带的诱导伴随着DNA的去除。单链特异性核酸酶S1和脱氧核糖核酸酶I不会产生这种条带模式。
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