Cunningham G R, Tindall D J, Huckins C, Means A R
Endocrinology. 1978 Jan;102(1):16-23. doi: 10.1210/endo-102-1-16.
The hormonal and testicular effects of hemicastration have been examined using rodent models. When rats were hemicastrated at 5 days of age, significant testicular hypertrophy was noted within 5 days. Hypertrophy decreased as the age at hemicastration approached 20 days and did not occur in rats 45 days of age or older. The changes in testicular weight were associated with significant (P less than 0.001) increases in serum FSH values from 10-20 days, but no significant alterations in the intratesticular concentration of testosterone occurred. Hemicastration also caused significant hypertrophy in testes depleted of germ cells. This was reflected by a substantial increase in testicular protein and DNA when compared to intact controls at 10, 15, and 20 days of age; however, the concentration of protein/microgram DNA was increased only at 15 days. These data indicate that 1) changes in serum FSH and not intratesticular testosterone are associated with the testicular hypertrophy which follows unilateral castration of immature animals; 2, a significant proportion of the hypertrophy can be attributed to non-germinal cells, the Sertoli cell being the prime candidate;3) the increase in testicular weight is primarily the result of an increase in cell number.
已使用啮齿动物模型研究了半侧睾丸切除的激素和睾丸效应。5日龄大鼠进行半侧睾丸切除后，5天内即出现明显的睾丸肥大。随着半侧睾丸切除时年龄接近20天，肥大程度降低，45日龄及以上的大鼠未出现肥大。睾丸重量的变化与10至20天血清促卵泡激素（FSH）值显著（P小于0.001）升高相关，但睾丸内睾酮浓度未发生显著改变。半侧睾丸切除还导致生殖细胞耗竭的睾丸显著肥大。这在10、15和20日龄时与完整对照相比，睾丸蛋白质和DNA大幅增加中得到体现；然而，仅在15日龄时蛋白质/微克DNA浓度增加。这些数据表明：1）血清FSH的变化而非睾丸内睾酮与未成熟动物单侧去势后出现的睾丸肥大相关；2）很大一部分肥大可归因于非生殖细胞，支持细胞是主要候选细胞；3）睾丸重量增加主要是细胞数量增加的结果。
