Weisz J, O'Brien L V, Lloyd T
Endocrinology. 1978 Jan;102(1):330-3. doi: 10.1210/endo-102-1-330.
A partially purified preparation of hydroxy-indole-O-methyltransferase (HIOMT) from bovine pineal was shown to O-methylate estradiol-17beta (E2). The HIOMT preparation was incubated with E2 and [3H]-S-adenosylmethionine (SAM). The [3H]-3 methyl-ether or estradiol [MeO-E2] produced was identified by thin layer chromatography (TLC) and crystallization to constant 3H/14C ratio in the presence of [14C]-MeO-E2 and unlabeled MeO-E2 standards. The kinetics of the enzyme reaction for melatonin formation from N-acetyl-serotonin (NAS) and for MeO-E2 were compared.
从牛松果体中提取的部分纯化的羟基吲哚 - O - 甲基转移酶（HIOMT）制剂被证明可使雌二醇 - 17β（E2）发生O - 甲基化。将HIOMT制剂与E2和[³H] - S - 腺苷甲硫氨酸（SAM）一起孵育。通过薄层色谱法（TLC）以及在[¹⁴C] - MeO - E2和未标记的MeO - E2标准品存在下结晶至恒定的³H/¹⁴C比值，来鉴定产生的[³H] - 3 - 甲醚或雌二醇[MeO - E2]。比较了由N - 乙酰 - 5 - 羟色胺（NAS）形成褪黑素以及形成MeO - E2的酶反应动力学。
