Bello J, Patrzyc H
Int J Pept Protein Res. 1980 May;15(5):464-70. doi: 10.1111/j.1399-3011.1980.tb02922.x.
The fluorogenic amine reagent 4-chloro-7-nitrobenzofurazan (CNBFz) reacts with amines to give one of two products, or a mixture, depending on the concentration of amine. At low concentrations (e.g. 5 X 10(-4) M) of unprotonated amine the normal replacement of the chlorine occurs, giving lambdamax approximately 470 nm. At high concentration of unprotonated amine (e.g. 0.5 M), a new product, lambdamax approximately 380 nm, is produced. At intermediate concentrations, both products are seen. These results were obtained with aqueous ammonia, methylamine and butylamine, and with butylamine in dry hexane. The 380 nm product also is obtained with high local concentrations of amines, such as with [lys]n at pH 8.5-10 and micellar dodecylamine at pH 9.5. These results are suggested to be analogous to those of very primitive proto-enzyme systems, by which clusters of small molecules can produce quantitative changes in "metabolism". Reaction of CNBFz with gelatin gave qualitative evidence for cross-linking.
荧光胺试剂4-氯-7-硝基苯并呋喃（CNBFz）与胺反应会生成两种产物之一或混合物，具体取决于胺的浓度。在低浓度（例如5×10⁻⁴ M）的未质子化胺条件下，会发生氯的正常取代反应，产生的最大吸收波长约为470 nm。在高浓度的未质子化胺（例如0.5 M）条件下，会生成一种新产物，其最大吸收波长约为380 nm。在中等浓度时，两种产物都会出现。这些结果是使用氨水、甲胺和丁胺以及在干燥己烷中的丁胺得到的。在高局部浓度的胺（例如在pH 8.5 - 10的[赖氨酸]ₙ和pH 9.5的胶束十二烷基胺）条件下也能得到380 nm的产物。这些结果被认为类似于非常原始的原酶系统的结果，通过该系统小分子簇可以在“代谢”中产生定量变化。CNBFz与明胶的反应给出了交联的定性证据。
