4-氯-7-硝基苯并呋咱的高亲电特性及其作为蛋白质标记试剂应用的可能后果。
The highly electrophilic character of 4-chloro-7-nitrobenzofurazan and possible consequences for its application as a protein-labelling reagent.
作者信息
Baines B S, Allen G, Brocklehurst K
出版信息
Biochem J. 1977 Apr 1;163(1):189-92. doi: 10.1042/bj1630189.
Abstract
4-Chloro-7-nitrobenzofurazan possesses at least one highly electrophilic centre (C-6) that is much more reactive towards nucleophiles than position C-4, the irreversible alkylating site of this reagent. Possible consequences of the electrophilic character of 4-chloro-7-nitrobenzofurazan for its application as a protein-labelling reagent are discussed.
摘要
4-氯-7-硝基苯并呋咱具有至少一个高亲电中心（C-6），该中心对亲核试剂的反应性比该试剂的不可逆烷基化位点C-4高得多。讨论了4-氯-7-硝基苯并呋咱的亲电特性对其作为蛋白质标记试剂应用的可能影响。
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本文引用的文献
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Investigation of the active site of papain with fluorescent probes.用荧光探针研究木瓜蛋白酶的活性位点。
Biochem J. 1973 Aug;133(4):679-86. doi: 10.1042/bj1330679.
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