Niehaus G D, Schumacker P T, Saba T M
J Appl Physiol Respir Environ Exerc Physiol. 1980 Oct;49(4):693-9. doi: 10.1152/jappl.1980.49.4.693.
Lung microvascular permeability in sheep increases during Pseudomonas bacteremia. The sheep's low plasma opsonic fibronectin concentration and associated inefficient reticuloendothelial clearance of blood-borne particulates may contribute to the response of increased lung vascular permeability during sepsis. The present study investigated the influence of sepsis on lung fluid balance in sheep with and without opsonic glycoprotein (plasma fibronectin) deficiency. Using the lung lymph fistula preparation in sheep, we made measurements of lung lymph flow (QLYM), lymph-to-plasma protein concentration ratios (L/P), pulmonary hemodynamics, and extravascular lung water content. Deficiency of opsonic fibronectin resulted in a minimal increase in lymph flow with no change in transvascular protein clearance (QLYM X L/P). Pseudomonas sepsis with or without fibronectin deficiency resulted in a stable L/P and a transient increase in pulmonary arterial pressure, which declined to a new steady state. Although sepsis resulted in a 100% elevation (P < 0.05) in lymph flow and transvascular protein clearance, sepsis in the presence of fibronectin deficiency induced a sustained 300--400% increase in lymph flow and a 300% increase in transvascular protein clearance. Thus opsonic fibronectin deficiency exaggerates the increased lung vascular permeability during sepsis.
在铜绿假单胞菌菌血症期间，绵羊的肺微血管通透性增加。绵羊血浆调理素纤连蛋白浓度较低，以及相关的对血源颗粒的网状内皮清除效率低下，可能导致脓毒症期间肺血管通透性增加的反应。本研究调查了脓毒症对有或无调理素糖蛋白（血浆纤连蛋白）缺乏的绵羊肺液体平衡的影响。利用绵羊的肺淋巴瘘制备，我们测量了肺淋巴流量（QLYM）、淋巴与血浆蛋白浓度比（L/P）、肺血流动力学和血管外肺水含量。调理素纤连蛋白缺乏导致淋巴流量略有增加，而跨血管蛋白清除率（QLYM×L/P）无变化。有或无纤连蛋白缺乏的铜绿假单胞菌脓毒症导致L/P稳定，肺动脉压短暂升高，随后降至新的稳定状态。尽管脓毒症导致淋巴流量和跨血管蛋白清除率升高100%（P<0.05），但存在纤连蛋白缺乏时的脓毒症导致淋巴流量持续增加300%-400%，跨血管蛋白清除率增加300%。因此，调理素纤连蛋白缺乏会加剧脓毒症期间肺血管通透性的增加。
