Moler T L, Donahue S E, Anderson G B, Bradford G E
J Anim Sci. 1980 Aug;51(2):300-3. doi: 10.2527/jas1980.512300x.
The effect of fetal and maternal genotype on embryo survival was studied in two lines of mice from the same base population: line E, selected for high embryo survival, and line CN-, selected for small litter size. Embryos were collected at 3.5 days postcoitus from normally mated donors of both lines and were transferred to the uteri of 2.5-day pseudopregnant recipients from each line. The number of embryos recovered and the proportion of those recovered that had been fertilized were greater in line E donors than in line CN- donors (P < .05). Development into blastocysts was slower for CN- embryos. The proportion of recipient females that were pregnant was higher for line E than for line CN- after transfer of line E embryos (P < .005). The same trend was observed following transfer of line CN- embryos (P < .10). Embryos of both lines showed greater survival when transferred to line E recipients than when transferred to line CN- recipients (P < .005), and, within line E recipients, E embryos tended to survive better than did CN- embryos (P < .10). The results suggest that although the genotype of the embryo may play a role, the maternal genotype has greater influence on embryo survival in the two lines of mice.
品系E，为高胚胎存活率而选育；品系CN-，为小窝产仔数而选育。在交配后3.5天从两个品系正常交配的供体中收集胚胎，并将其移植到来自每个品系的2.5天假孕受体子宫中。品系E供体中回收的胚胎数量以及回收的已受精胚胎比例高于品系CN-供体（P <.05）。CN-胚胎发育成囊胚的速度较慢。移植品系E胚胎后，品系E受体雌性怀孕的比例高于品系CN-（P <.005）。移植品系CN-胚胎后也观察到相同趋势（P <.10）。两个品系的胚胎移植到品系E受体时比移植到品系CN-受体时显示出更高的存活率（P <.005），并且在品系E受体中，E胚胎的存活率往往比CN-胚胎更好（P <.10）。结果表明，虽然胚胎基因型可能起作用，但在这两个品系小鼠中母体基因型对胚胎存活率的影响更大。
