Nonaka K, Sasaki Y, Martin D A, Nakata M
Department of Pediatric Dentistry, Kyushu, University, Faculty of Dentistry, Fukuoka, Japan.
J Assist Reprod Genet. 1995 Aug;12(7):447-52. doi: 10.1007/BF02211146.
The effects of dam strain on the spontaneous incidence of the cleft lip and palate (CLP) and the intrauterine growth of transferred CLP-susceptible CL/Fr embryos were examined with an embryo transfer technique. The CL/Fr strain of embryos at the early blastocyst stage was transferred to the same dam strain and also to the CLP-resistant C57BL dam strain. A laparotomy was done on the 18th gestational day at which time the number of fetuses, the resorption sites and the fetus weight were recorded. Each fetus was checked for the presence of CLP. Five criteria to assess the reproduction and fertility as well as the fetus weight were then compared between both dam strains.
The dam pregnancy rate and the fetus survival rate in the CL/Fr dam strain were both significantly lower than those in the C57BL dam strain. The resorption rate in the CL/Fr dam strain was significantly higher than that in the C57BL dam strain. The spontaneous incidence rate of CLP in the CL/Fr dam strain was also significantly higher than that in the C57BL dam strain. The fetus weight of the CL/Fr fetuses developed in the CL/Fr dam strain was significantly lighter than that of the CL/Fr fetuses developed in the C57BL dam strain.
The results indicated that the CLP-susceptible CL/Fr dam strain provided a less favorable uterine environment for the implantation, survival and intrauterine growth of the transferred CL/Fr embryos and also caused a higher spontaneous incidence rate of CLP. Thus, it can be concluded that the effect of the dam strain appears to play an important role on the spontaneous incidence of CLP and the intrauterine growth of the CL/Fr strain embryos transferred to both CL/Fr and C57BL dam strains.
采用胚胎移植技术，研究母鼠品系对唇腭裂（CLP）自发发生率以及移植的CLP易感性CL/Fr胚胎宫内生长的影响。将早期囊胚阶段的CL/Fr品系胚胎移植到同品系母鼠以及CLP抗性C57BL母鼠体内。在妊娠第18天进行剖腹手术，记录胎儿数量、吸收部位和胎儿体重。检查每个胎儿是否存在唇腭裂。然后比较两种母鼠品系在繁殖力、生育力以及胎儿体重这五个评估标准方面的差异。
CL/Fr母鼠品系的母鼠妊娠率和胎儿存活率均显著低于C57BL母鼠品系。CL/Fr母鼠品系的吸收率显著高于C57BL母鼠品系。CL/Fr母鼠品系中唇腭裂的自发发生率也显著高于C57BL母鼠品系。在CL/Fr母鼠品系中发育的CL/Fr胎儿体重显著轻于在C57BL母鼠品系中发育的CL/Fr胎儿。
结果表明，CLP易感性CL/Fr母鼠品系为移植的CL/Fr胚胎的着床、存活和宫内生长提供了不太有利的子宫环境，并且还导致唇腭裂的自发发生率更高。因此，可以得出结论，母鼠品系的影响似乎对唇腭裂的自发发生率以及移植到CL/Fr和C57BL母鼠品系中的CL/Fr品系胚胎的宫内生长起着重要作用。
