Mashimo J, Yoshida M, Ikeuchi K, Hata S, Arata S, Kasai N, Okuda K, Takazoe I
Microbiol Immunol. 1985;29(5):395-403. doi: 10.1111/j.1348-0421.1985.tb00840.x.
The composition and the nature of the linkage of fatty acids and the Shwartzman activity of lipopolysaccharide (LPS) preparations derived from oral gram-negative bacteria including Bacteroides gingivalis, Bacteroides loesheii, Eikenella corrodens, Fusobacterium nucleatum, and Actinobacillus actinomycetemcomitans were examined. 3-Hydroxylated and nonhydroxy fatty acids of various chain lengths were found in all of the LPS preparations. All nonhydroxy fatty acids were found to be ester-bound, and part of the 3-hydroxy fatty acids in the LPS of B. gingivalis, E. corrodens, F. nucleatum, and A. actinomycetemcomitans were shown to be involved in ester linkage. It was also suggested that the hydroxy group of the ester-bound 3-hydroxy fatty acid of the LPS of F. nucleatum and A. actinomycetemcomitans is at least partly substituted by another fatty acid, but in the LPS of B. gingivalis and E. corrodens it is not. The main amide-linked fatty acid of the LPS of B. gingivalis, E. corrodens, F. nucleatum, and A. actinomycetemcomitans was 3-hydroxyheptadecanoic, 3-hydroxydodecanoic, 3-hydroxyhexadecanoic, and 3-hydroxytetradecanoic acid, respectively. The results of the Shwartzman assay showed that the E. corrodens LPS was the most active among the preparations tested, and that the Shwartzman toxicity of Bacteroides LPS is extremely low.
对来源于口腔革兰氏阴性菌（包括牙龈卟啉单胞菌、洛氏卟啉单胞菌、腐蚀艾肯菌、具核梭杆菌和伴放线放线杆菌）的脂多糖（LPS）制剂中脂肪酸的组成、连接性质及其施瓦茨曼活性进行了检测。在所有LPS制剂中均发现了各种链长的3-羟基化脂肪酸和非羟基化脂肪酸。所有非羟基化脂肪酸均为酯键结合，并且牙龈卟啉单胞菌、腐蚀艾肯菌、具核梭杆菌和伴放线放线杆菌LPS中的部分3-羟基化脂肪酸显示参与酯键连接。还表明，具核梭杆菌和伴放线放线杆菌LPS中酯键结合的3-羟基化脂肪酸的羟基至少部分被另一种脂肪酸取代，但牙龈卟啉单胞菌和腐蚀艾肯菌的LPS中则不然。牙龈卟啉单胞菌、腐蚀艾肯菌、具核梭杆菌和伴放线放线杆菌LPS的主要酰胺键连接脂肪酸分别为3-羟基十七烷酸、3-羟基十二烷酸、3-羟基十六烷酸和3-羟基十四烷酸。施瓦茨曼试验结果表明，在测试的制剂中，腐蚀艾肯菌LPS活性最高，而卟啉单胞菌LPS的施瓦茨曼毒性极低。
