Reaves T A, Hayward J N
J Comp Neurol. 1980 Oct 1;193(3):777-88. doi: 10.1002/cne.901930313.
We report the development of a double-label method which combines intracellular recording, dye-marking, and immunocytochemistry for the study of functional and morphological aspects of peptide-containing neurons in the magnocellular preoptic nucleus of the goldfish hypothalamus. Using multiple techniques, we distinguish three types of peptide-containing neurons: enkephalin (27%), isotocin (49%), and vasotocin (24%). Drawings of serial sections containing the dye-injected cells were the basis for subsequent reconstruction. These peptide-containing cells measure 14-56 micrometer in somata diameter, are unipolar, bipolar, or multipolar in somata shape and lie 6-244 micrometer from the ependymal lining of the preoptic recess of the third ventricle. Their electrophysiological properties match those of other mammalian and fish magnocellular neuroendocrine cells. This report confirms the one neuron, one hormone (peptide) hypothesis, supports synaptic over electrotonic coupling between peptidergic and adjacent hypothalamic neurons, and suggests that chemical and functional classification of single electrophysiologically and neuroanatomically studied central neurons is feasible technologically.
我们报告了一种双标记方法的开发，该方法结合了细胞内记录、染料标记和免疫细胞化学，用于研究金鱼下丘脑大细胞视前核中含肽神经元的功能和形态学方面。通过多种技术，我们区分出三种含肽神经元：脑啡肽（27%）、异催产素（49%）和血管催产素（24%）。包含染料注射细胞的连续切片绘图是后续重建的基础。这些含肽细胞的胞体直径为14 - 56微米，胞体形状为单极、双极或多极，位于第三脑室视前隐窝室管膜衬里6 - 244微米处。它们的电生理特性与其他哺乳动物和鱼类的大细胞神经内分泌细胞相匹配。本报告证实了一个神经元、一种激素（肽）的假说，支持肽能神经元与相邻下丘脑神经元之间的突触连接而非电紧张偶联，并表明对单个经电生理和神经解剖学研究的中枢神经元进行化学和功能分类在技术上是可行的。
