Kawata M, Sano Y, Inenaga K, Yamashita H
Histochemistry. 1983;78(1):21-6. doi: 10.1007/BF00491107.
An attempt was made to identify Lucifer Yellow-labeled neurons in the rat supraoptic nucleus as vasopressin-containing neurons, by means of a combination of immunoperoxidase histochemistry and iontophoretic single cell-injection. We came to the conclusion that the fluorescent dye does not diminish the immunoreactivity of vasopressin in the magnocellular neurons. This newly developed method, along with its modifications, should prove to be quite useful for electrophysiological and morphological studies on the neuropeptide-releasing neurons in the mammalian neuroendocrine system.
通过免疫过氧化物酶组织化学和离子电渗单细胞注射相结合的方法，试图将大鼠视上核中用荧光黄标记的神经元鉴定为含加压素的神经元。我们得出的结论是，荧光染料不会降低大细胞神经元中加压素的免疫反应性。这种新开发的方法及其改进方法，对于哺乳动物神经内分泌系统中神经肽释放神经元的电生理和形态学研究应该会非常有用。
