Reaves T A, Hayward J N
Proc Natl Acad Sci U S A. 1979 Nov;76(11):6009-11. doi: 10.1073/pnas.76.11.6009.
A method that combines intracellular recording, dye marking, and immunocytochemistry makes the study of functional and morphological aspects of enkephalin neurons in the magnocellular preoptic nucleus of the goldfish hypothalamus feasible. By use of multiple techniques, enkephalin neurons can be distinguished from other brain cells and can be reconstructed from drawings of serial sections containing the dye-injected opioid cells. These enkephalin cells and their processes measure 14-42 micron in somata diameter and are unipolar, bipolar, or multipolar. Their electrophysiological properties match those of other mammalian and fish magnocellular endocrine cells. This report confirms the one neuron-one hormone (peptide) hypothesis, supports synaptic over electronic coupling between enkephalin and adjacent hypothalamic neurons, and suggests that chemical and functional classification of single electrophysiologically and neuroanatomically studied central neurons can be achieved.
一种将细胞内记录、染料标记和免疫细胞化学相结合的方法，使得对金鱼下丘脑大细胞视前核中脑啡肽能神经元的功能和形态学方面进行研究成为可能。通过使用多种技术，可以将脑啡肽能神经元与其他脑细胞区分开来，并可以根据包含注射了染料的阿片样物质细胞的连续切片的绘图进行重建。这些脑啡肽能细胞及其突起的胞体直径为14 - 42微米，呈单极、双极或多极。它们的电生理特性与其他哺乳动物和鱼类的大细胞内分泌细胞相匹配。本报告证实了一个神经元一种激素（肽）的假说，支持脑啡肽与相邻下丘脑神经元之间的突触联系而非电偶联，并表明可以对单个经过电生理学和神经解剖学研究的中枢神经元进行化学和功能分类。
