Lang D R, Kurzepa H, Cole M S, Loper J C
J Environ Pathol Toxicol. 1980 Aug;4(1):41-54.
Malignant transformation of BALB/3T3 1-13 cells results following treatment with complex mixtures of residue organic chemicals from drinking water samples of five U. S. cities. The U. S. Environmental Protection Agency provided the residues which were obtained by liquid-liquid solvent extraction, and sorption-desorption on XAD-2 resin of samples concentrated by reverse osmosis from drinking water of Philadelphia, PA; New Orleans, LA; Miami, FL; Seattle, WA; and Ottumwa, IA. Transformation was observed using a focus formation assay following 72-hr exposure of cells to residues from one or more samples from each city. Malignancy of several transformed and normal clones was evaluated in athymic (nu/nu) mice. The usefulness of such a transformation assay in detecting potential carcinogenic compounds contained in complex mixtures and the evaluation of possible health effects of residue organics in drinking water are discussed.
用来自美国五个城市饮用水样本中的残留有机化学物质的复杂混合物处理后，BALB/3T3 1-13细胞发生恶性转化。美国环境保护局提供了这些残留物，它们是通过液-液溶剂萃取以及对宾夕法尼亚州费城、路易斯安那州新奥尔良、佛罗里达州迈阿密、华盛顿州西雅图和爱荷华州奥塔姆瓦饮用水经反渗透浓缩后的样本在XAD-2树脂上进行吸附-解吸得到的。在细胞暴露于来自每个城市一个或多个样本的残留物72小时后，使用集落形成试验观察到转化。在无胸腺（nu/nu）小鼠中评估了几个转化克隆和正常克隆的恶性程度。讨论了这种转化试验在检测复杂混合物中潜在致癌化合物以及评估饮用水中残留有机物可能的健康影响方面的实用性。
