Zagris N
Exp Cell Biol. 1985;53(4):233-9. doi: 10.1159/000163316.
Clusters of 20-70 erythroblasts from blood islands of early chick blastoderm were cultured in serum-free chemically defined medium for a 3-month period. The erythroblast cluster produces erythroid cells and hemoglobins characteristic of the primitive and definitive erythroid cell lines. It seems there is a progenitor erythroid cell(s) in the erythroblast cluster which starts and/or continues maturing along various pathways of hemopoietic differentiation under simple culture conditions. The erythroid character of these cells is stable during the 3-month culture period.
从早期鸡胚囊胚的血岛中分离出的20 - 70个成红细胞簇,在无血清化学限定培养基中培养3个月。成红细胞簇产生原始和定型红细胞系特有的红细胞和血红蛋白。似乎在成红细胞簇中存在祖红细胞,其在简单培养条件下沿着造血分化的各种途径开始和/或继续成熟。在3个月的培养期内,这些细胞的红细胞特征是稳定的。